Transcriptional analysis using E13.5 mouse eyes of TGFb2/GDNF mutants compared to WT

Eyes of E13.5 mouse embryos of TGFb2/GDNF knock-out mutant and wildtype (both identified by genotyping) were dissected and gene expression was compared by microarray. Coloboma were found in the mutant mice, and the time-point E13.5 was chosen to address transcriptional changes during optic fissure closure. The knock-out mutant was created from the cross of TGFbeta and GDNF single mutants (Sanford et al 1997 and Pichel et al 1996).