Transcriptional analysis using E13.5 mouse eyes of TGFb2/GDNF mutants compared to WT

Eyes of E13.5 mouse embryos of TGFb2/GDNF knock-out mutant and wildtype (both identified by genotyping) were dissected and gene expression was compared by microarray. Coloboma were found in the mutant mice, and the time-point E13.5 was chosen to address transcriptional changes during optic fissure closure. The knock-out mutant was created from the cross of TGFbeta and GDNF single mutants (Sanford et al 1997 and Pichel et al 1996).本研究对TGFβ2/GDNF双敲除突变体与野生型（均经基因分型鉴定）的E13.5小鼠胚胎眼球实施解剖分离，采用微阵列（microarray）技术对二者的基因表达谱进行比较分析。突变小鼠中可观察到眼裂缺损（coloboma），选取E13.5作为实验时间点，旨在解析视裂闭合过程中的转录组变化。该双敲除突变体通过TGFβ2与GDNF单突变体杂交构建而成（Sanford等，1997；Pichel等，1996）。