scRNA-seq of microglia from Cx3cr1CreERT2(Litt) adult mice reveals gene expression profile after TAM treatment.

The Cx3cr1CreERT2(Litt) mouse model is widely used for in-depth studies of myeloid cells, including microglia. In this study, we employed single-cell RNA sequencing (scRNA-seq) to characterize microglial responses to tamoxifen (TAM) administration in adult Cx3cr1CreERT2(Litt) mice. Our analysis revealed a prolonged TAM-dependent induction of CDKN1A in the microglial population, highlighting a effect of the Cre-induction system itself. Transcriptome profiles of microglia from whole brains of Cx3cr1-CreERT2(Litt) mouse models with TAM-treatment were generated, following the 10x Gemomics protocol.