Transcriptomic profile at single cell level of TIL-NK cells and tumor cells from tumor-bearing Reg-1fl/fl and Reg-1ΔNK mice

The innate immune system can recognize tumor cells and coordinate with the adaptive immune system to initiate an anti-tumor response within the tumor microenvironment. To investigate the role of NK cells and their interaction with other immune cells in the anti-tumor immunity in Reg-1ΔNK. We subcutaneously injected B16F10 cells into Reg-1fl/fl and Reg-1ΔNK, followed by scRNA-seq analysis of the whole tumor cell suspension and sorted TIL-NK cells. B16F10 cells were s.c. injected into Reg-1fl/fl and Reg-1ΔNK mice (Day 0). Tumor-bearing mice were sacrificed on Day 10 for Reg-1fl/fl mice and Day 17 for Reg-1ΔNK mice, respectively. Tumor suspension and sorted NK cells from tumor-bearing Reg-1fl/fl and Reg-1ΔNK mice were analyzed using scRNAseq