X-ray Structure of the H181N/E224Q double mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and dTDP

X-ray Structure of the H181N/E224Q double mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and dTDP Descriptor: 1,2-ETHANEDIOL, S-ADENOSYL-L-HOMOCYSTEINE, THYMIDINE-5'-DIPHOSPHATE, ... Authors: Bruender, N.A, Holden, H.M. Deposit date: 2012-03-09 Release date: 2012-04-25 Last modified: 2023-09-13 Method: X-RAY DIFFRACTION (1.5 Å) Cite: Probing the catalytic mechanism of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitrose. Protein Sci., 21, 2012

TcaB9的H181N/E224Q双突变体的X射线晶体结构，该蛋白属于C-3'-甲基转移酶（C-3'-Methyltransferase），其与S-腺苷-L-同型半胱氨酸（S-Adenosyl-L-Homocysteine）及脱氧胸腺嘧啶核苷二磷酸（dTDP）形成复合物。配体描述：1,2-乙二醇（1,2-ETHANEDIOL）、S-腺苷-L-同型半胱氨酸、胸腺嘧啶-5'-二磷酸（THYMIDINE-5'-DIPHOSPHATE）等。作者：Bruender, N.A.、Holden, H.M.。提交日期：2012-03-09；发布日期：2012-04-25；最后修改日期：2023-09-13。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.5埃（1.5 Å）。引用文献：《探究参与D-四硝糖（D-tetronitrose）生物合成的C-3'-甲基转移酶的催化机制》，发表于《Protein Science》，2012年，第21卷。