Bulk RNA-seq was performed on murine Lin-Sca+Kit+ (LSK) bone marrow cells from a transgenic SCLtTA/BCR-ABL (DTG) mouse BM cells (CD45.2) in which BCR-ABL1 was expressed or not expressed

Bulk RNA-seq was performed on murine Lin-Sca+Kit+ bone marrow cells from a transgenic SCLtTA/BCR-ABL (DTG) mouse BM cells (CD45.2) to understand the molecular consequences of BCR-ABL1 expression on the global mRNA programme of these cells. Overall design: Eight to twelve week old B6.SJL recipient mice (CD45.1) were irradiated with two doses of 4.25Gy, 3hrs apart and transplanted with 2 x 106 SCLtTA/BCR-ABL (DTG) mouse BM cells (CD45.2) via tail vein injection. Tetracycline (TET) was included in the drinking water for two weeks post-transplant to allow time for recovery and engraftment. Two weeks post irradiation, TET was removed for two weeks in one cohort, allowing BCR-ABL1 expression and leukaemia development (n=6; CML group), while TET administration was continued In a second cohort (n=6; CTRL group). RNA was extracted and libraries made for RNA-seq analysis. Both male and female age-matched recipient mice were used for this study.