Gene expression profiling of dynamic paracrine senescence in human fibroblasts

Cellular senescence can be transmitted to neighbouring cells in a paracrine manner through different mechanisms, including soluble factors released by senescent cells. To understand the dynamic regulation of paracrine senescence, here we investigated gene expression profiles in normal human fibroblasts (IMR90) exposed to conditioned medium generated by an inducible model of fibroblast Oncogene-Induced Senescence (IMR90-ER:RAS) at different time points after induction of senescence. mRNA expression profiles were obtained from human IMR90 primary fibroblasts exposed to conditioned medium from IMR90-ER:RAS fibroblasts at different times after senescence induction with 4-hydroxy-tamoxifen (4-OHT). Each sample was analyzed in duplicate by RNA-Seq using Illumina HiSeq.