Influence of IWS1 TND-interacting motifs (TIMs) on gene expression patterns [RNA-seq]

To reveal the influence of TND-interacting motifs (TIMs), which bind TFIIS N-terminal domains (TNDs), we prepared KELLY cells expressing either wild-type IWS1-FLAG or IWS1-FLAG containing disruptive mutations in the unstructured TIM3 region. Following lentiviral transduction of either WT or M3 IWS1-FLAG, we prepared mRNA from these samples and performed expression profiling using RNA-seq. Analysis of differential gene expression provided markers that are differentially regulated by TIM3 of IWS1. Overall design: RNA-seq of KELLY cells expressing wild-type (WT) or mutant (M3) IWS1-FLAG via lentiviral transduction.