seurat_object_V3.RData

Chemotherapy have been widely applied to the comprehensive treatment of lung adenocarcinoma (LUAD) patients, but its effect on immune microenvironment of lung adenocarcinoma needs further study. We obtained a total of 83622 cells derived from the tumor samples of nine LUAD patients who received only surgery or neoadjuvant chemotherapy. Functional enrichment analysis revealed that chemotherapy drove significant metabolic reprogramming in tumor cells and macrophages, which explained its enhanced malignancy and tumor-promoting phenotype after neoadjuvant treatment. Moreover, through analyzing the remodeling of T and B cells induced by neoadjuvant therapy, we noted that chemotherapy ignited a relatively stronger immune cytotoxic response towards tumor cells. Besides, a series of marker genes of different cell types and driver genes motivating phenotype shifting, such as metabolic reprogramming, were identified. Also, we constructed cell-to-cell crosstalk atlas based on tumor and immune cells at the single-cell level. In summary, our study demonstrates the metabolic reprogramming within the TME of LUAD induced by chemotherapy.