Metabolomics and stable 13C isotope tracing in circulating hemocytes during parasitoid wasp infection.

MS Excel sheets with stable 13C isotope tracing experiments. List of metabolites, their characterizations, identification, HPLC/HRMS parameters, and compensation of natural occurrence is on the first sheet [S2a_List of Metabolites]. Data from the following experiments are in individual sheets (values are raw or normalized areas under respective chromatographic peaks with compensation of natural occurrence in yellow-highlighted cells marked by * in headings): [S2b_13C in vivo]—raw and normalized data from hemocytes obtained 22 h after start of infection from uninfected and infected w1118 larvae fed a diet with 50% D-Glucose-13C6 for the last 6 h. [S2c_13C ex vivo] raw and normalized data from hemocytes obtained 22 h after start of infection from uninfected and infected Srp>P{y[+t7.7] = CaryP}attP2 control larvae and incubated for 40 min in medium containing 5 mM unlabeled trehalose and 0.5 mM 13C6 labeled glucose or 5 mM 13C12 labeled trehalose and 0.5 mM unlabeled glucose. [S2d_13C ex vivo Trehcs1], [S2e_13C ex vivo Trehc1], and [S2f_13C ex vivo TrehRAdG4] sheets—raw data from hemocytes obtained 22 h after start of infection from uninfected and infected w1118 control and Trehcs1, Trehc1, or TrehRAΔG4 mutant larvae and incubated for 40 min in medium containing 5 mM 13C12 labeled trehalose and 0.5 mM unlabeled glucose. [S2g_13C ex vivo Srp-Tret1-1-RNAi] raw and normalized data from hemocytes obtained 22 h after start of infection from uninfected and infected Srp>P{y[+t7.7] = CaryP}attP2 control larvae and larvae with hemocyte-specific Tret1-1 RNAi Srp>P{TRiP.HMS02573}attP2 and incubated for 40 min in medium containing 5 mM 13C12 labeled trehalose and 0.5 mM unlabeled glucose. Raw data are available at figshare (https://doi.org/10.6084/m9.figshare.25525657.v1). (XLSX)