Expression analysis in IBM maize roots

Roots of young plants undergo highly regulated gene expression changes that pattern root architecture and physiology, with lifelong effects on the structural integrity, water-use efficiency, and nutrient flow of the plant. To understand gene regulatory networks in maize roots, we measured transcript levels in two-week-old roots of 218 greenhouse-grown lines belonging to the maize Intermated B73 x Mo17 (IBM) recombinant inbred population. After performing quality control on the root RNA-seq data, we retained an average of 19.6 million reads per sample. Sample identity was verified by calling SNPs on the RNA-seq data to compare with SNPs used in previous work with this population (Asaro et al. 2016). Samples were considered validated if the new SNPs matched previous SNPs with an accuracy above 90% once low accuracy SNPs (

幼苗的根系在生命历程中经历着高度调控的基因表达变化，这些变化塑造了根系的形态结构和生理机能，并对植物的结构完整性、水分利用效率和营养流动产生终身影响。为了解玉米根系中的基因调控网络，我们对属于玉米互交B73 x Mo17（IBM）重组自交群体中218个温室种植玉米品种两周龄根系中的转录水平进行了测量。在对根系RNA-seq数据进行质量控制后，我们保留了每个样本平均19.6百万个读数。通过在RNA-seq数据上调用SNPs并与之前对该群体进行的研究中使用的SNPs进行比较，验证了样本身份。当新发现的SNPs与先前SNPs的匹配精度高于90%时，样本被认为已通过验证（Asaro等，2016年）。