Genetic diversity at the two time points for the Cape mountain zebra in the three reserves, Mountain zebra National Park (MZNP), De Hoop Nature Reserve (DHNR) and Kammanassie Nature Reserve (KNR).

Population genetic diversity over for two periods spanning around 16 years (1999–2001 to 2015–2016) was evaluated based on allelic richness (Ar), observed heterozygosity (Ho) as well as unbiased expected Heterozygosity (He) and fixation (FIS) across 11 polymorphic microsatellite loci. Detection of recent bottlenecks were tested by identifying whether Mountain Zebra National Park had significant levels of heterozygous excess using the Two Phase Mutation model (Wilcoxon PTPM and PSMM) and then confirmed by checking allelic mode shift at mutation-drift equilibrium (ADIST). * and bold text = significant temporal change, p < 0.05. 1indicates estimates of Ar are corrected for sample size through rarefaction in HP-RARE using the smallest number of gene copies per population (MZNP = 14, DHNR = 24, KNR = 4) and 2indicates were analysis was not applicable, since sample size was too small for KNR and in DHNR, the population is an admixture of MZNP and KNR.