Next-generation sequencing analysis to compare expression pattern of relapse and baseline "GMP pattern" MDS samples

Transcriptional changes occurring in the expanding LMPP population of "GMP pattern" MDS patients with blast expansion post-HMA failure were compared with the transcriptional profile of untreated (baseline) "GMP pattern" MDS patients. CD34+CD38-CD45RA+CD90- LMPPs were FACS-purified from magnetically CD34-enriched mononuclear cells isolated by density gradient from fresh bone marrow aspirates. Total RNA was purified using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific). Quality checks were performed in a 2100 Expert bioanalyzer (Agilent). cDNA libraries were prepared using the Ovation Single Cell RNA-Seq System (NuGEN Technologies). RNA-seq was performed with the HiSeq 4000 System (Illumina), generating 76-base pair reads. **The submitter declares that the raw data will be deposited in dbGaP due to patient privacy concerns.**