Dermal fibroblast cultures recapitulate differences between deermice and mice in responses to a Toll-like receptor agonist

The white-footed deermouse Peromyscus leucopus is a primary reservoir for the agents of Lyme disease and other zoonoses in North America. These and other species of Peromyscus are tolerant of the infection by the bacteria, protozoa, and viruses they host. This is mainly achieved by mitigating the damaging effects of the innate immune response. In previous studies, we demonstrated differences between P. leucopus and either inbred or outbred M. musculus in the degree of sickness and profiles of biomarkers after exposure to bacterial lipopolysaccharide, a TLR4 agonist. To develop a method for broadly assessing of innate immunity of capture-release mammals in nature, we evaluated using bulk and single-cell RNA-seq primary dermal fibroblast cultures of P. leucopus and M. musculus in their short-term responses to a lipopeptide that is a TLR2 agonist. As we had observed for experimental animals, the fibroblast cultures of the two species displayed both similarities and differences in their res..., , # Dermal fibroblast cultures recapitulate differences between deermice and mice in responses to a Toll-like receptor agonist

Dataset DOI: [10.5061/dryad.m905qfvdq](10.5061/dryad.m905qfvdq)

## Description of the data and file structure

Peromyscus leucopus and Mus musculus RNA-seq data of coding sequences (CDS), endogenous retroviruses (ERVs) and species comparisons across treatment groups of control or 1 µg/ml or 10 µg/ml Pam3CSK4. 

### Files and variables

#### File: TableD6_PM_ERV_100k_FC.xlsx

**Description:** Table D6. ERV/TEs ≥500 bp of P. leucopus and M. musculus dermal fibroblasts without (control) or with 1 µg/ml Pam3CSK4 and by length, transcription, and fold change of treatment to control.

Column names: ERV ≥500, genome location, length (bp), mean transcription control, mean transcription 1 µg/ml Pam3CSK4, log2 fold chain (FC). 

#### File: TableD1_Peromyscus_CDS_22K.xlsx

**Description:** Table D1. Peromyscus leucopus dermal fibroblast normalized RNA-seq reads per kb of g...,