Brain-wide imaging in flp-13 animals upon PA14 infection

When animals are infected by a pathogen, peripheral sensors of infection signal to the brain to coordinate a set of adaptive behavioral changes known as sickness behaviors. While the pathways that signal from the periphery to the brain have been intensively studied in recent years, how central circuits are reconfigured to elicit these behavioral changes is not well understood. Here, we find that neuromodulatory systems linked to stress and satiety are recruited during chronic pathogen infection to alter the behavior of C. elegans. Upon chronic infection by the bacterium Pseudomonas aeruginosa PA14, C. elegans decrease their feeding behavior, then display reversible bouts of quiescence, and eventually die. The ALA neuron and its neuropeptides FLP-7, FLP-24, and NLP-8, which control stress-induced sleep in uninfected animals, promote the PA14-induced feeding reduction. However, the ALA neuropeptide FLP-13 instead acts to delay quiescence and death in infected animals. Cell-specific geneti..., , , # Whole-brain Imaging data structure documentation

Each of the 8 animals is summarized in a h5 file. All h5 files follow an identical structure.

## Overview

The h5 files contain various measurements and analyses related to neural and behavioral data, including body angles, velocities, neural traces, and timestamps.

## Data Structure

### Behavioral Measurements

* `velocity`: Axial velocity measurements in mm/s
* `pumping`: Pharyngeal pumping rate measurements in Hz
* `θh`: Head angle measurements in radian
* `θh_pos_is_ventral`: Boolean flag indicating if a positive value in the head angle measurement corresponds to ventral
* `ang_vel`: Angular velocity measurements in rad/s
* `body_angle`: Angles between the first 30 regularly interspaced keypoints along the midline of the worm
* `curve`: Worm curvature, as defined by the standard deviation of body_angles at each time point
* `s_velocity`, `s_θh`, `s_pumping`, `velocity_s`, `θh_s`, `pumping_s`: normalized behavioral measurements t...