E. coli DHFR complex with NADP+ and 10-methylfolate

E. coli DHFR complex with NADP+ and 10-methylfolate Descriptor: Dihydrofolate reductase, MANGANESE (II) ION, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... Authors: Greisman, J.B, Brookner, D.E, Hekstra, D.R. Deposit date: 2022-06-13 Release date: 2023-06-21 Last modified: 2024-11-13 Method: X-RAY DIFFRACTION (1.14 Å) Cite: Perturbative diffraction methods resolve a conformational switch that facilitates a two-step enzymatic mechanism. Proc.Natl.Acad.Sci.USA, 121, 2024大肠杆菌（E. coli）二氢叶酸还原酶（Dihydrofolate reductase, DHFR）与烟酰胺腺嘌呤二核苷酸磷酸（NADP+）及10-甲基叶酸的复合物。样品组成：二氢叶酸还原酶（Dihydrofolate reductase）、锰(II)离子、烟酰胺腺嘌呤二核苷酸磷酸（NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE）…… 作者：Greisman, J.B.、Brookner, D.E.、Hekstra, D.R. 提交日期：2022-06-13 发布日期：2023-06-21 最后修改日期：2024-11-13 实验方法：X射线衍射（1.14 Å） 引用文献：《扰动衍射法解析调控两步酶促机制的构象开关》，《美国国家科学院院刊（Proc. Natl. Acad. Sci. USA）》，2024年，第121卷。