LHFPL3-AS2 lncRNA regulates epithelial polarity and its reduced expression is linked with more severe Crohn Disease

LHFPL3-AS2 knockdown Caco-2 cells (g1 and g2) and controls (gCtl) Overall design: A loss-of-function (LOF) system in Caco-2, We used the CRISPR interference (CRISPRi) system and two independent LHFPL3-AS2 specific guide RNAs (g1 and g2 gRNAs), which depleted LHFPL3-AS2 expression as confirmed by qPCR in comparison to control gRNA.