Reorganization of Lamina Associated Domains in early mouse embryos is regulated by RNA Polymerase II activity.

How the earliest events in embryogenesis, including zygotic genome activation (ZGA) during maternal-to-zygotic transition influence chromatin remodelling remains unknown. Here, we have investigated the establishment of nuclear architecture, focusing on the remodelling of Lamina Associated Domains (LADs) during this transition. We report that LADs reorganize gradually in 2-cell embryos and that blocking ZGA leads to major changes in nuclear organisation. Overall design: LaminB1 DamID in wildtype early 2-cell embryos and in late 2-cell embryos either treated with alpha-amanitin or DRB.