Transcriptome network analysis of Smug1 knock-out cells by using CRISPR/Cas9 system

Purpose: To build a differential transcriptome network in Smug1 knock-out HepG2 hepatocarcinoma cells. Methods: Transcriptome analysis by the RNA-seq via mRNA pull-down. Results: We constructed transcriptome from Smug1 knock-out cells by using RNA-seq analysis. Nucleosome and miRNA related genes were highly enriched in Smug1 KO HepG2 cells. Conclusions: Smug1 regulate the gene expression related with nucleosome assembly and histone function. The start codon region of Smug1 gene was targeted for CRISPR/Cas9 to generate Smug1 KO HepG2 cells. After generate KO cells, we isolated total RNA from KO and WT HepG2 cells and performed directional mRNA-sequencing.