Raman spectra as recorded for the RNA structures of 20 different SARS-CoV-2 variants/subvariants

RNA Raman spectra were collected by means of a high spectrally resolved spectrometer designed for measurements on biological samples (LabRAM HR800, Horiba/Jobin-Yvon, Kyoto, Japan). The equipment was set in con-focal mode with a 20x objective lens and the wavelength of the incoming light was 532 nm, as generated by a solid-state laser source operating at 10 mW. The Raman scattered light was monitored by means of a single mono-chromator interfaced with an air-cooled charge-coupled device (CCD) detec-tor (Andor DV420-OE322; 1024 × 256 pixels). The acquisition time for a single spectrum was typically 20 s for three successive acquisitions at each location. A spectral resolution better than 1 cm^-1 could be achieved by con-currently collecting (during each measurement) an internal reference signal from a selected neon lamp to calibrate eventual scattering of the spectrometer. Series of 10 spectra were systematically collected at different locations (over a total area of ~2 mm²) for each RNA sample and averaged in order to obtain statistically representative spectra. Data within selected spectral areas were also collected in the same way. The correspondence between file names and virus strain names is as follows:s01: JPN/TY/WK-521s02: QHN001 (α)s03: TY8-612 (β)s04: TY7-503 (γ)s05: TY11-927 (δ)s06: TY33-456 (λ)s07: TY28-444 (θ)s08: TY26-717 (µ)s09: TY38-873 (ο)s10: TY38-871 (ο)s11: TY40-385 (ο)s12: TY41-721 (ο)s13: TY41-716 (ο)s14: TY41-703 (ο)s15: TY41-763 (ο)s16: TY41-702 (ο)s17: TY41-704 (ο)s18: TY41-796 (ο)s19: TY41-795 (ο)s20: TY41-686 (ο)