mGBP2 interacting proteins during Toxoplasma gondii infection

Guanylate binding proteins (GBPs) are a family of large interferon-inducible GTPases that execute essential host defense activities against a wide variety of intracellular microbial pathogens. Toxoplasma gondii is an invasive intracellular apicomplexan protozoan parasite of global importance. Murine GBPs (mGBPs) recognize T. gondii PVs and assemble into supramolecular mGBP homo- and heterocomplexes that are required for the disruption of the membrane of PVs eventually resulting in the cell-autonomous immune control of vacuole-resident pathogens. Here, in order to unravel mGBP2 functions, we characterized mGBP2 protein interactions partners in cells with and without Toxoplasma gondii infection by an AP-MS approach.