Nanodisc reconstituted MsbA in complex with nanobodies, spin-labeled at position A60C

Nanodisc reconstituted MsbA in complex with nanobodies, spin-labeled at position A60C Descriptor: (1~{R},4~{R},11~{S},14~{S},19~{Z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione, (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-4-[(3~{R})-3-nonanoyloxytetradecanoyl]oxy-5-[[(3~{R})-3-octanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{S},5~{S},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanylnonanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-oxan-2-yl]methoxy]-5-oxidanyl-oxane-2-carboxylic acid, ATP-dependent lipid A-core flippase, ... Authors: Januliene, D, Parey, K, Galazzo, L, Meier, G, Vecchis, D, Striednig, B, Hilbi, H, Schaefer, L.V, Kuprov, I, Bordignon, E, Seeger, M.A, Moeller, A. Deposit date: 2021-08-16 Release date: 2022-08-24 Last modified: 2025-07-09 Method: ELECTRON MICROSCOPY (3.7 Å) Cite: The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells. Sci Adv, 8, 2022纳米圆盘（Nanodisc）重构的与纳米抗体（nanobodies）结合的MsbA蛋白，在A60C位点进行了自旋标记（spin-labeled）。描述符：(1R,4R,11S,14S,19Z)-19-[2-[2,5-二氧代吡咯烷-1-基]乙亚胺]-7,8,17,18-四氧杂-1,4,11,14-四氮杂三环[12.6.2.2^4,11]二十四烷-6,9,16-三酮；(2R,4R,5R,6R)-6-[(1R)-1,2-二羟基乙基]-4-[(2R,3S,4S,5R,6R)-6-[(1S)-1,2-二羟基乙基]-4-[(2R,3S,4S,5S,6R)-6-[(1S)-1,2-二羟基乙基]-3,4,5-三羟基氧杂环己烷-2-基]氧基-3,5-二羟基氧杂环己烷-2-基]氧基-2-[[(2R,3S,4R,5R,6R)-4-[(3R)-3-壬酰氧基十四酰基]氧基-5-[[(3R)-3-辛酰氧基十四酰基]氨基]-6-[[(2R,3S,4S,5S,6R)-3-羟基-5-[[(3R)-3-羟基壬酰基]氨基]-4-[(3R)-3-羟基十四酰基]氧基-6-膦酰氧基氧杂环己烷-2-基]甲氧基]-3-膦酰氧基氧杂环己烷-2-基]甲氧基]-5-羟基氧杂环己烷-2-羧酸；ATP依赖型脂质A-核心翻转酶……作者：Januliene D、Parey K、Galazzo L、Meier G、Vecchis D、Striednig B、Hilbi H、Schaefer L.V、Kuprov I、Bordignon E、Seeger M.A、Moeller A。存档日期：2021年8月16日；发布日期：2022年8月24日；最后修改日期：2025年7月9日。实验方法：电子显微镜（分辨率3.7 Å）。引用文献：ABC转运蛋白（ABC transporter）MsbA在大肠杆菌（E. coli）细胞中呈广泛向内开放构象。《Science Advances》，8卷，2022年