Bacteria cells in the surface water of the Mackenzie Delta Region during 4 expeditions from spring to fall in 2019

Samples for bacterial abundance (1.5 mL) were preserved with glutaraldehyde (1% final concentration) and stored at -80°C. Samples were stained with SYBRTM Green I (Thermofisher Scientific) and analyzed on a flow cytometer (FACSCanto, BD Biosciences) as previously described (Gasol & Del Giorgio, 2000; doi:10.3989/scimar.2000.64n2197).用于细菌丰度检测的1.5 mL样本采用终浓度为1%的戊二醛固定，并于-80℃低温保存。样本经SYBR™ Green I（赛默飞世尔科技公司，Thermofisher Scientific）染色后，参照Gasol与Del Giorgio于2000年发表的方法（DOI:10.3989/scimar.2000.64n2197），使用BD Biosciences公司生产的FACSCanto流式细胞仪进行分析。