wt and sua7-1 nascent mRNA profiling in relation to termination of transcription

wt and sua7-1 nascent mRNA profiling was performed using the GRO-seq approach. Br-UTP labelling of nascent RNA followed by immunoprecipitation and cDNA library preparation with sequencing (peformed exactly as described in O'Brien et al., 2023). Three biological replicates were performed. Overall design: wt and sua7-1 cells were cultured, nascent RNA was labelled, immunoprecipitated, cDNA library asssembled, and sequenced.