KEGG pathway enrichment analysis.

Therapeutic resistance remains a primary obstacle to curing cancer. Healthy cells exposed to genotoxic insult rapidly activate both p53-dependent and -independent non-genetic programs that pause the cell cycle and direct either DNA repair or apoptosis. Cancer cells exploit these same pathways as they respond to stresses induced by cancer therapies. In this study, we investigated a potential role for upstream stimulatory factor 1 (USF1) and USF2 in the p53-independent response of lymphoma cells to genotoxic therapy. We previously found that lymphocytes utilize the responsiveness of USF1 to double-stranded DNA breaks to coordinate T cell receptor beta (Tcrb) gene expression during V(D)J recombination. Here, microarray gene expression analysis of derivatives of the p53-deficient mouse B lymphoma cell line, M12, revealed that simultaneously depleting cells of both USF1 and USF2 altered the expression of 940 gene transcripts (>1.50-fold change,