AFLPseq Nanopore FASTQ read data Leontodon sect. Asterothrix (Cass.) Ball (Compositae, Cichorieae)
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https://www.ncbi.nlm.nih.gov/sra/SRP618055
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The here presented multi-locus Nanopore read data comprises 36 Leontodon sect. Asterothrix (Cass.) Ball (Compositae, Cichorieae) samples. The samples were prepared for sequencing using the AFLPseq genome-reduction method as proposed by Dorfner et al. (2022, DOI: https://doi.org/10.1002/aps3.11484), with the addition of conducting a double digest approach using the restriction enzymes MseI and EcoRI and the 'Ligation by Sequencing LSK114' protocol by Oxford Nanopore Technologies. With the SLANG pipeline (Dorfner et al. 2022), 2,681 orthologous loci were inferred, containing 12,183 SNPs. After calculation of Jukes-Cantor distances, a Neighbor-Net network reveals two poles consisting of crispus/biscutellifolius and hellenicus/valeriae, with asper holding an intermediate position between these two poles. In combination with morphological data, we propose the treatment of asper as a subspecies of L. crispus, crispus/biscutellifolius as two varieties of L. crispus subsp. crispus, and hellenicus/valeriae as two subspecies of L. hellencius:Leontodon crispus subsp. crispus var. crispusL. crispus subsp. crispus var. biscutellifoliusL. crispus subsp. asperLeontodon hellenicus subsp. hellenicusL. hellenicus subsp. valeriae
创建时间:
2025-09-17



