RNA-seq of precision cut liver slice (PCLS) culture before slicing and up to 9 days after slicing, changing media either every day or every 3 days, with insulin

This experiment is designed to test the effect of culture time, media change regime, and insulin on liver slices. Samples were taken before slicing and 3, 4, 5, 6, 7, 8, and 9 days after slicing. Samples had media changed either every day or every three days and insulin was included in the media at 20nM concentration.