Neuronal deformation data and cellular morphology data from 'High-speed interferometric imaging reveals dynamics of neuronal deformation during the action potential'

This dataset contains the experimental data presented in the paper 'High-speed interferometric imaging reveals dynamics of neuronal deformation during the action potential'.Neuronal deformations during the action potential on six different cells and the corresponding cellular morphology data are provided in this dataset. The neuronal deformations were recorded by quantitative phase microscopy (QPM) with spike-triggered averaging synchronized to optogenetic stimulation. The QPM data represents the optical path length changes through the neurons at 10 kHz sampling rate, with filtering and corrections applied as described in the publication. The cellular morphology of each cell in the QPM dataset was captured via confocal microscopy using the same fluorophore used for the optogenetic control. The confocal image stacks represent a close approximation to the underlying cellular morphology of the cells whose deformations were recorded at the much higher axial and temporal resolution in QPM.The deformations associated with the action potential and morphology data together should provide a useful framework for those studying the electro-mechanical coupling in neurons and other electrically active cells. Electrical and structural models of the membrane deformations can be simulated on the morphological data and compared to the measured deformations.Please refer to the paper for more details on the imaging method and results.