Buruli ulcer (BU), caused by infection with Mycobacterium ulcerans, is a chronic necrotizing human skin disease associated with the production of the cytotoxic macrolide exotoxin mycolactone. Despite
a6–8 weeks old mice were infected i.n. with either F. tularensis LVS or the indicated mutants and monitored for mortality for 28 days. Data are shown as number of mice survived/total number of mice in
Pools of mutants of minimal complexity but maximal coverage of genes of interest facilitate screening for genes under selection in a particular environment. We constructed individual deletion mutants
WT = Comparison of gene expression in wild type inoculated loop versus uninfected control loops.MUT = Comparison of gene expression in ΔsipA, sopABDE2 mutant inoculated loops versus uninfected control
