Effect of SMARCA4 knockdown on repeat elements in senescent IMR90 ER:RAS cells

We report the analysis of RNA sequencing aimed at understanding the effects induced by the knockdown of SMARCA4 on the expression of repeat and transposable elements in senescent cells. SMARCA4 was initially identified as a target through a genetic screening process designed to locate regulators responsible for upregulating SASP. SMARCA4 is a constituent of the SWI/SNF complex subunit. The findings will help characterise he expression of repeat elements to elucidate the pathway by which SMARCA4 knockdown can promote cytokine expression and Natural Killer cell recruitment. Overall design: IMR90 ER:RAS cells were transfected with siRNAs against target genes and non-targeting scramble controls (by reverse transfection) one day prior to senescence induction. Oncogene-induced senescence was induced following the administration of 4OHT (tamoxifin) treatment. RNA from the cells was extracted seven days following senescence induction.