Chromatin remodeling in bovine oocytes and preimplantation embryos

Profiles of H3K4me3, H3K27ac, H3K27me3 and H3K9me3 in bovine GV oocytes and preimplantation embryos, and the characterization of chromatin accessibility in bovine blastocyst, inner cell mass and trophectoderm. Overall design: H3K4me3, H3K27ac, H3K27me3 and H3K9me3 were profiled with CUT&Tag technique in bovine GV oocytes (GV), 2-cell embryos (2C), 4-cell embryos (4C), 8-cell embryos (8C), morula (MO), blastocyst (BL), inner cell mass (ICM) and trophectoderm (TE). To investigate the relationship between embryonic transcription and chromatin remodeling, embryos were concurrently cultured in the presence of a transcriptional inhibitor (a-amanitin) and a-amanitin-treated 8-cell embryos (8A) were collected for library construction of H3K4me3, H3K27ac, H3K27me3 and H3K9me3. ATAC-seq libraries were prepared from bovine blastocyst, inner cell mass and trophectoderm.