Fig. 3e-f | Quantitative immunofluorescence for TDP-43 localization in motor neuron lines

This item is part of the Figshare Project:Early mitochondrial dysfunction revealed across FUS- and TARDBP-ALS at single cell resolutionFrom Data Availability Statement for the forthcoming paper in Nature Communications entitled:Single-cell RNA sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS- and TARDBP-ALS"We have deposited all raw and processed RNA sequencing data generated in this study on the NCBI Gene Expression Omnibus (GEO) under the accession number GSE226482. The C9orf72-ALS bulk RNA sequencing data was retrieved directly from the authors of the study.[Items under this Figshare Project contain:] "Scans of fluorescent western blots, raw imaging files from confocal microscopy, the analysis files from Opera Phenix, qPCR data sets, and Seahorse assay result files."--------------------------------------------[Item specific description:]Immunofluorescence microscopy of TDP-43 in in-vitro differentiated ALS motor neurons.These files are related to quantification of TDP-43 localization and phosphorylation in ALS motor neurons using the Opera Phenix high content imaging system. More information here on our imaging platform: https://www.kclwcic.co.uk/operaphenixSample meta information and plate layouts are available as XLSX and PDF files.The file format is .czi, through the ZEN software (Zeiss). Zeiss recommends using ImageJ and the ImageJ-based Fiji software package.Information about the microscopy file format CZI can be found here: https://www.zeiss.com/microscopy/en/products/software/zeiss-zen/czi-image-file-format.html