3D landscape of Hepatitis B virus interactions with human chromatins [RNA-seq]

We employed a highly sensitive technology, 3C-HTGTS (3C-high-throughput genome-wide sequencing), to globally identify HBV DNA-host DNA contacts in cellular models of HBV infection. We found that HBV cccDNA does not randomly position in host genome, but instead preferentially establishes contacts with the host DNA at active chromatin regions. In contrast, the organization of integrated HBV DNA is largely regulated by the local genome epigenetic environment, which particularly forms chromosome loop with host genome where gene promoters together with active histone modifications are enriched. Our study provides a high throughput 3D landscape for the spatial organizations of cccDNA and integrated HBV DNA within the human genome, and provides a foundation to further understand the mechanisms of HBV modulation of liver disease development and progression in the future. Overall design: Examination of 2 different HBV DNAs in 2 HBV cell models using two different primer. HepAD38 replicates human hepatitis B virus (HBV) under conditions that can be regulated with tetracycline.