Effect of DUSP6 overexpression or knockdown on gene expression in AML12 cells

DUSP6 plays important roles in MAPK signaling pathway, but whether and how it is involved in liver funciton remains to be explored. Here, we performed RNA-seq analyses in AML12 cells where DUSP6 is disrupted. We overexpressed GFP-DUSP6 or GFP in AML12 cells, and tested the effects of DUSP6 increase on gene expression in AML12 cells. Meanwhile, we knocked down DUSP6 in AML12 cells and tested the effects of DUSP6 decrease on gene expression in AML12 cells. Taken together, we analyzed the changes of gene expression mediated by DUSP6, which provides new insights for the function of DUSP6 in liver Overall design: We transiently overexpressed GFP-DUSP6 or GFP in AML12 cells by lipo2000, and 24 hours later harvested cells for RNA-seq analyses. Meanwhile, we transfected AML12 cells with DUSP6-specific siRNA or negative-control (NC) siRNA by lipo2000, and 48 hours later harvested cells for RNA-seq analyses. Each group contains three samples.