Role of FTO in ovarian cancer (MeRIP-Seq)

RNA methylation plays an important role fine tuning translation and subsequently regulating cellular responses and cell fate. The fat mass- and obesity-associated protein (FTO) was recognized as an m6A demethylase and described as an oncogenic factor in leukemia and brain tumors. FTO expression levels are suppressed in ovarian tumors and ovarian cancer stem cells (CSCs). FTO induce cyclic AMP activity through targeting PDE4B and PDE1C by down-regulation of m6A levels in the mRNA transcript. In all or findings point to a tumor suppressor function of FTO in high grade serous OC GFP (control) or FTO-GFP (treatment) over-expression in OVCAR-5 cells 3 biological replicates for IgG input for GFP, 3 biological replicates for m6A pull-down for GFP, 3 biological replicates for IgG input for FTO-GFP, and 3 biological replicates for m6A pull-down for FTO-GFP