Supporting data for “Harnessing Chemical Proteomics to Uncover Readers of Novel Histone PTMs &Development of YEATS2-targeted Inhibitors and Degraders for Epigenetic Therapies”

In this thesis, we tried to use chemcial proteomics method to uncover the reader proteins of novel histone post translational modifications. At first, we performed experiment to find some potential protein, thus we generated MS data search (As shown in H4K12ac and H4K12acme), in this data, we could select some candidate for our following experiment. Also, we further validated this result by western blot (As shown in WB summary). Also, since the reader proteins serve as attractive drug target, we also tried to develop PROTACs targeting the reader proteins. Through the rational design, we obtained several compounds for the following experiment, to test the efficiency of our candidates, we validated its degradation efficiency through western blot again (As shown in WB summary). In the WB summary, it showed different degradation effect of our product, through this data analysis, we can concluded that most of product did not show high potency, which might require further optimization.