Thermophilic Geobacillus WSUCF1 secretome for saccharification of AFEX and EA pretreated corn stover

It is difficult to identify individual hydrolyzing enzymes using routine enzyme assays in the laboratory, which only give a collective picture of the secretome enzymatic activity. A proteomic analysis method based on liquid chromatography followed by tandem mass spectrometry (LCMS/MS) has proven significant for profiling CAZymes in secretome and is a powerful tool to identify enzymes that are otherwise impossible to detect. Furthermore, proteomic analysis of secretome against genomic data using different bioinformatics tools provides more details about CAZymes and relative quantifications of enzymes in each sample.