Number of genes up-regulated and down-regulated as an effect of laboratory adaption in male and female 845b houseflies.

Sequences which were found in less than ten copies in F1 flies were considered background noise. This left 19,756 and 19,150 sequences for males and females, respectively. Values of F13 and F29 above 1.2-fold F1 fly expression were considered up-regulated, while values below 0.8-fold F1 were considered down-regulated. Values above 0.8-fold and below 1.2-fold were characterized as ‘unchanged’ from the F1 flies.