Transient dietary intervention induces healthy adipose tissue expansion and metabolically healthy obesity in mice

As obesity progresses, dynamic tissue remodeling of adipose tissue occurs over time, i.e., adipocyte hypertrophy, chronic inflammation, and interstitial fibrosis. Some obese individuals exhibit healthy adipose tissue expansion, characterized by modest inflammation and fibrosis despite adipocyte hypertrophy, resulting in “Metabolically Healthy Obesity (MHO)”. Our research has revealed that MHO can be induced by temporary weight loss during the development of obesity. We fed high-fat diet (HFD) to eight-week-old male C57BL/6J mice for 8 weeks (pre weight loss; preWL), followed by standard diet for 2 weeks (WL1w, WL2w) for weight loss We collected epididymal adipose tissue samples from the mice.