Expression Profiling of MicroRNAs in Relation to EGFR Mutation status in Japanese Smoker Male with Lung Adenocarcinoma [adinocarcinoma tissue]

The lung adenocarcinoma has an increased incidence in smokers. The molecular target therapy has been developed for lung adenocarcinoma patients harboring EGFR gene mutation. However, the treatment options for the patients without mutation are currently limited. Thus, analysis of EGFR gene mutation status at early stage is important strategy to classify patients for effective treatments and improving prognosis. This study aimed to identify microRNA (miRNAs) signature in relation to mutation status in EGFR gene in early stage of lung adenocarcinoma male patients with smoking history. MiRNA profiles were assessed by microarray in paired plasma and tissue pooled from 10 EGFR wild type (EGFR-wt) and 10 EGFR mutated (EGFR-mut) patients. Total RNA containing small RNA was pooled equally for each 10 lung adenocarcinoma tissues from EGFR-wt and EGFR-mut patients respectively for microarray analysis. MiRNA profiling was examined using a Toray 3D-Gene® miRNA oligo chip Human miRNA version 21 (Toray, Tokyo, Japan) on which 2,565 probes were mounted. The expression level of each miRNA was normalized using the median signal strength for the entire gene in each chip.