The redox structure of haem- and flavoproteins by combining X-ray diffraction and UV-vis and Raman spectroscopy

The overall aim of the project is deciphering reaction mechanisms of selected haem- and flavoproteins. These systems are very labile for radiation damage of the redox sites during diffraction data collection, which makes in situ studies using single-crystal UV-vis and Raman spectroscopy inevitable to both prove and trap different redox states and to monitor the potential radiation damage. The project will especially focus on obtaining the true structures of different redox states of the proteins myoglobin and flavodoxin, through a composite and multicrystal data collection approach monitored with spectroscopy.