Identified downstream binding partners of SmTK4.

Clones identified by Y3H library screening using the linker region combined with the TK-domain of SmTK4 as bait and separately the TK domain of SmTK3 (SmTK4-linker+TK + SmTK3-TK pBridge). From the 72 initial prey clones obtained following library screening (mating) 19 clones remained positive after further selection procedures. From these clones the preys were isolated, their inserts sequenced, and after BLASTX analyses arranged into five groups (A–E) due to their homologies. The last group contains the clones showing no significant homology in the BLAST analyses (group E). The other four groups represent proteins with homology to small HSPs as well as to the schistosome major egg antigen Smp40 (group A), a caspase 3 (group B), a MAPK-activating protein (group C), and a leucine-rich protein with homology to mapmodulin (group D). To depict the e-values of the candidate clones, of which only partial sequences were obtained from the Y2H library, the full-length cDNA sequences (fl) of the clones from group A–D were identified in the genome data set of the S. mansoni sequencing project ([6]; www.sanger.ac.uk, www. genedb.org), or in the NCBI database (www.ncbi.nlm.nih.gov) and used for BlastX analyses. The sizes of the full-length sequences, the appropriate e-values, and the clone numbers are given.