Data from 'Loss of coral thermotolerance following year-long in situ nursery propagation with a consecutively high summer heat-load.'

Exposure to more frequent ocean warming events is cumulatively driving the loss of coral reef health as the window of recovery between episodes of bleaching reduces. Coral propagation via in situ nurseries and subsequent out-planting practices have increased worldwide to support replenishing coral cover on degraded reefs. However, challenges in identifying fast-growing and bleaching-resistant target corals have limited how informative we can be regarding the resilience of out-planted corals. Here, we employed short-term thermal stress assays to assess the thermal threshold of a fast-growing coral pre- and post-propagation on in situ nursery frames. We show that year-long nursery propagated corals exhibit a significant reduction in thresholds compared to their corresponding donor colonies, likely due to the more intense summer heat-loading experienced during the propagation phase just prior to stress testing. Data files provided: 'adapters.fasta' contains adapters used by Trimmomatic to target removal of illumina adapters from raw reads. 'ahyc_oist_v1.0.zip' contains the Acropora hyacinthus reference genome, gff and gtf file used for the RNASeq analysis (originally sourced from /marinegenomics.oist.jp) 'CBASSnursery_ITS2_SymPortal_output.zip' contains all SymPortal (https://symportal.org) output files. Note, this SymPortal run contained results also of samples from other experiments, refer to only sampled named Ah-1 (mislabelled, refers to Ah-2 in manuscript), Ah-4, Ah-8, Ah-11, Ah-17. Raw fastq files available at: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1011835 Publication: Alderdice R, Voolstra CR, Lendo CIN, Boote C, Suggett DJ, Edmondson J, et al. Loss of coral thermotolerance following year-long insitu nursery propagation with a consecutively high summer heat-load. Coral Reefs. 2024. doi:10.1007/s00338-024-02505-9 Scripts for analysis available at: https://github.com/RachelCAlderdice/CBASS_Nursery