Tissue Specimens from Patients with Melanoma for Genetic Analysis

The purpose of this study was to obtain tissue specimens derived from patients with melanoma to generate research tools to advance our understanding of the genetics, pathogenesis, and therapeutics of melanoma. Briefly, tissue was obtained from metastatic lesions and used to generate clonal primary cell lines from melanoma cells and fibroblasts from the tumor microenvironment. RNA was extracted from low passage cell lines using Trizol reagent. cDNA libraries were prepared using the TruSeq mRNA sample preparation kit, v2 (Illumina) and sequenced on the HiSeq 2000 platform (Illumina). The submitted files are bam files that contain both unaligned and aligned reads (human genome, build hg19). ]]>
Collection of Tissue Specimens and Clinical Data from Patients with Melanoma for Genetic AnalysisCases: This protocol is designed to collect specimens from new and existing patients seen in the cutaneous oncology program at Dana-Farber Cancer Institute and Brigham and Women's Hospital. Subjects must have histologically confirmed metastatic melanoma. Subjects should not need to undergo any procedures outside of the standard of care to obtain specimens. All tissue will have already been collected or will be collected as part of a biopsy being performed for standard of care management. No surgical or invasive procedures will be performed specifically for the purposes of this study.]]>