Variations of lymphocyte populations 72

C  =  Control, R  =  Restrained, HU  =  Hindlimb Unloaded.
* p  =  0.025 versus C. ¤p  =  0.081 versus C.
After 72 hours of culture, cells were incubated with fluorescent antibodies before identification by flow cytometry. Specific subpopulations of lymphocytes were determined within the lymphocyte gate. The formula [(% population stimulated - % population unstimulated)/% population unstimulated] was used to calculate the percentage of variation after stimulation with mitogens. Each group was compared to the others. n  =  5 mice per group. Statistically significant differences were found using an ANOVA and Tukey post-hoc tests. After LPS stimulation, the number of total lymphocytes was increased by only 16.9% in the hindlimb unloaded (HU) group compared to 41.6% in control (C) mice, corresponding to a reduction of 60% that was significantly different (p  =  0.025 versus C). Data are mean values ± SEM.