Sequencing muscle short RNAs (16-40nt) to investigate possible regulation of myogenesis phenotypes.. Mus musculus strain:C3H

Short RNAs (sRNAs) originating in pseudogenes and retrotransposons have been demonstrated to function as trans-regulatory elements in mammals. It followed therefore that the observed myogenesis phenotypes may have been caused by the perturbation of LINE-1 derived and/or other sRNAs, which are known to affect numerous cellular processes including differentiation. We therefore sequenced 2.1 million 16-40nt sRNAs from C2C12 muscle cells from one of the broadly used mouse strains.