Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography–Tandem Mass Spectrometry

We performed proteomic analyses of
human olfactory epithelial tissue to identify missing proteins using
liquid chromatography–tandem mass spectrometry. Using a next-generation
proteomic pipeline with a < 1.0% false discovery rate at the peptide
and protein levels, we identified 3731 proteins, among which five
were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434).
We validated the identified missing proteins using the corresponding
synthetic peptides. No olfactory receptor (OR) proteins were detected
in olfactory tissue, suggesting that detection of ORs would be very
difficult. We also identified 49 and 50 alternative splicing variants
mapped at the neXtProt and GENCODE databases, respectively, and 2000
additional single amino acid variants. This data set is available
at the ProteomeXchange consortium via PRIDE repository (PXD010025).