UHPLC-MS/MS analysis of S-nitrosylated proteins involved in autophagy in Triticum aestivum roots

In the present work, we analyzed the patterns of protein S-nitrosylation during the inductionof autophagy in сommon wheat (Triticum aestivum) roots. S-nitrosylated proteins were accumulated in plant tissues as a result of artificially induced autophagy, visualized usingWestern blot and identified by bottom-up proteomics methods. Further, the obtained data was analyzed by bioinformatics methods to predict possible S-nitrosylation sites and protein-protein interactions between S-nitrosylated proteins and possible targets. These resultscontribute to the development of the efficient protocols for identification and analysis of S-nitrosylated proteins in plants.