S1 File - Sedimentary DNA is a promising indicator of the abundance of marine benthos: Insights from the burrowing decapod Upogebia major

S1 Table. The total sedDNA concentration (ng/µL) of in each sample. BRA: burrow-rich areas, Lowercase letters (a–d) represent transects, and numbers indicate sampling locations (distance from 0 m point) for sediment. S2 Table. Results of the validation of the optimal annealing temperature in qPCR. Experimentation was conducted twice at temperatures of 60°C, 58°C, and 55°C, with the results indicating that 55°C yielded the highest detection count. S3 Table. The number of burrows per quadrat and the total number of burrow openings from 2021 to 2023. The blank areas indicate months where no survey was conducted. S4 Table. The numerical values indicating the reaction efficiency in qPCR. S5 Table. Copy numbers and sedDNA concentration data (Copies/g sediment). BRA: burrow-rich areas, Lowercase letters (a–d) represent transects, and numbers indicate sampling locations (distance from 0 m point) for sediment. S6 Table. Test results for sedDNA concentration between the burrow-rich area and other areas (A to D transects), as well as between each transect, using the Tukey–Kramer test (Q Value (α == 0.05)). S7 Table. The results of monthly sedDNA concentration comparisons within the burrow-rich area and across each transect, using the Tukey-Kramer test (Q value, α == 0.05). (XLSX)