Main cell populations fractions within non activated or PHA-activated human PBMCs at 24, 48 and 72 h after H-1 PV infection.

p.i: post-infection; NA: non activated PBMCs; A: activated PBMCs; MOI: multiplicity of infection.Freshly isolated PBMCs were inoculated with increasing amounts of H-1 PV or mock-treated. For each condition, cells were labelled with appropriate antibodies and the fraction of each cell population was analyzed using flow cytometry. Results are expressed as percentages of total cells within PBMCs. CD3+CD4+, CD3+CD8+, CD19+CD20+ and CD3+CD56+ respectively indicate the % of CD4+ helper T cells, CD8+ effector T cells, B cells and NK cells within PBMCs. CD3+CD69+ and CD3+CD30+ respectively indicate the % of early and late activated T cells within PBMCs. Representative data for all markers.