Gene Targeting Strategies and Screenings for Clones Having Undergone Targeted Integration Events
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https://figshare.com/articles/dataset/A_Role_for_PCNA_Ubiquitination_in_Immunoglobulin_Hypermutation/152752
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Physical maps of the loci, the targeting vectors, and the targeted alleles are shown for PCNA (A), RAD18 (B), and REV1 (C), respectively. The position and orientation of the primers used for the screening by long-range PCR are indicated. The identification of the desired clones relied on the appearance of new PCR fragments as well as the disappearance of germline fragments. �� HindIII/��X HaeIII was used as size marker. (366 KB PDF)
创建时间：
2015-12-02
